Cell Bio Assignments for Next Wednesday

• WPR Corrections (30 points). Append to your WPR #2 the corrections and citations. This does not need to be pretty, but it does need to be legible. E.g. On problem 3 the answer should be “D”. Taq is the DNA polymerase from a thermophilic organism and it’s used because the protein won’t denature/unfold at high temperatures in the PCR reaction. Source: lesson 16 slides and lecture and the DNAtube movie from the lesson objective handout.
• Central Dogma Assignment (30 points, 3 problems, 10 each): Start with each of the following DNA and show how it undergoes semi-conservative replication, the transcription of each product, and the translation of each product just as we did in problem H on the WPR (mutations in DNA 1 is a G-to-A transition and in DNA 2 is a depurination):

• Mobility Assignment (30 points): In your own handwriting, draw a combined figure of Figures 15-19 and 15-20 from the book. Include a brief but descriptive discussion on event sequence, affinity, specificity, and all the components including the lipid bilayer, adaptin, clathrin, dynamin, cargo receptor(s), cargo molecule(s), v-SNARE(s), t-SNARE(s). Ensure you label the cytosol side of a membrane and name the starting and ending compartments. Make it neat o/w your grade will reflect.