Wednesday Fun (or what you need to focus on)

These are the topics that you should really own during your prep for the exam:

• PCR – be able to talk me through a PCR reaction.
• Quiz #7 (not Restriction Enzyme problem)
• Restriction enzmes
• membranes – what they composed of, what crosses a lipid bilayer, what needs channels/pores
• Genetic control: DNA level
• Know the Trp Operon figure and be able to brief me on it
• Know the Lac Operon figure and be able to brief me on it
• Know the differences between DNA and RNA polymerases
• Know flavors of diffusion/transport across membranes
• Cytoskeleton: know the 3 types, the subunits, the polarity/direction, what marks + vs. – ends, what engines move toward + vs – ends.
• Know 1, 2, 3, and 4 structure in proteins.  Know the IMF and covalent interactions that drive these levels of structure.

Time will be an issue.  There will not be time to ponder my intent on wording etc – so no tricks, just up the middle definitions and concepts.

See me for AI if the Quiz 7 is not clear.

Good Luck.